Glucose-6-phosphate 1-dehydrogenase (30-1952)
Glucose-6-phosphate 1-dehydrogenase recombinant protein
Overview
Overview
| Synonyms | G6PD, G6PD1 |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
Specifications
| Expression System | Hi-5 cells |
|---|---|
| Grade & Purity | > 95% pure |
| Molecular Weight | 61.4kDa |
| Form & Buffer | Sterile Filtered colorless solution, containing 20mM Tris-HCl buffer (pH 8.0), 0.1mM PMSF, 2mM EDTA, 2mM DTT, 200mM NaCl and 20% glycerol. Please Note: Buffer may change with new Lots. |
Usage & Assay Information
| Bioactivity | Specific activity is > 7 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of bet |
|---|
Storage & Safety
| Storage | Store at 2-8 deg C for short term storage. For long term storage aliquot and store at -20 deg C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
|---|
General Information
| Biological Significance | Glucose-6-phosphate dehydrogenase is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. |
|---|---|
| Sequence Information | MGSSHHHHHH SSGLVPRGSH MAEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL LPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED FFARNSYVAG QYDDAASYQR LNSHMNALHL GSQANRLFYL ALPPTVYEAV TKNIHESCMS QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTN SDDVRDEKVK VLKCISEVQA NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAV VLYVENERWD GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH QIELEKPKPI PYIYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL |
Reviews
You need to be logged in to write a review. Please login here
Sorry there are currently no reviews for this product