Glucose Oxidase antibody (20R-GR007)
Rabbit polyclonal Glucose Oxidase antibody
Overview
Overview
| Synonyms | Polyclonal Glucose Oxidase antibody, Anti-Glucose Oxidase antibody, Glucose Oxyhydrase antibody, Glucose oxidase (Precursor) antibody, beta D Glucose Oxygen 1 Oxido Reductase antibody, GOD antibody |
|---|---|
| Specificity | Aspergillus niger glucose oxidase |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | Glucose oxidase antibody was raised in rabbit using glucose oxidase isolated from Aspergillus niger as the immunogen. |
Images
Western blot analysis using Glucose Oxidase antibody (20R-GR007)
Western Blot of Glucose Oxidase antibody. Lane 1: Glucose Oxidase. Lane 2: None. Load: 50 ng per lane. Glucose Oxidase antibody at 1:1,000 for overnight at 4 deg C. DyLight649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: Blocking buffer for 30 min at RT. Size: 80 kDa, 80 kDa for Glucose Oxidase from Aspergillus niger..
Specifications
| Host | Rabbit |
|---|---|
| Method of Purification | Glucose Oxidase antibody was purified by ion exchange chromatography followed by dialysis. |
| Form & Buffer | Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3. |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:35,000-1:160,000, IHC: 1:2,000-1:8,000, WB: 1:3,500-1:16,000 |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | The glucose oxidase enzyme (GOx) is an oxido-reductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono- Delta-lactone. In cells, it aids in breaking the sugar down into its metabolites. |
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