Glutathione reductase protein (His tag) (80R-2167)
Purified recombinant E.coli Glutathione reductase protein (His tag)
Overview
Overview
| Synonyms | gorA protein, Glutathione oxidoreductase protein, Gor protein, JW3467 protein, ECK3485 protein |
|---|---|
| Species | E.coli |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of Glutathione reductase protein (His tag) (80R-2167)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | MGSSHHHHHH SSGLVPRGSH MGSMTKHYDY IAIGGGSGGI ASINRAAMYG QKCALIEAKE LGGTCVNVGC VPKKVMWHAA QIREAIHMYG PDYGFDTTIN KFNWETLIAS RTAYIDRIHT SYENVLGKNN VDVIKGFARF VDAKTLEVNG ETITADHILI ATGGRPSHPD IPGVEYGIDS DGFFALPALP ERVAVVGAGY IAVELAGVIN GLGAKTHLFV RKHAPLRSFD PMISETLVEV MNAEGPQLHT NAIPKAVVKN TDGSLTLELE DGRSETVDCL IWAIGREPAN DNINLEAAGV KTNEKGYIVV DKYQNTNIEG IYAVGDNTGA VELTPVAVAA GRRLSERLFN NKPDEHLDYS NIPTVVFSHP PIGTVGLTEP QAREQYGDDQ VKVYKSSFTA MYTAVTTHRQ PCRMKLVCVG SEEKIVGIHG IGFGMDEMLQ GFAVALKMGA TKKDFDNTVA IHPTAAEEFV TMR |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 90% pure |
| Molecular Weight | 51.2 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Bioactivity | Specific activity: > 52 unit/ml. One unit will reduce 1.0 umol of oxidized glutathione per minute at pH 7.5 at 25C |
|---|
Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
|---|
General Information
| Biological Significance | Glutathione reductase belongs to the class-I pyridine nucleotide disulfide oxidoreductase family. The main function of the protein is to maintain high levels of reduced glutathione in the cytosol. With the concomitant oxidation of NADPH, Glutathione reductase transforms oxidized glutathione to the reduced form. The active site of the protein is a redox-active disulfide bond. Recombinant E. coli gor protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
|---|
Reviews
You need to be logged in to write a review. Please login here
Sorry there are currently no reviews for this product