Glyoxalase I antibody (70R-12932)

Affinity purified Rabbit polyclonal Glyoxalase I antibody

Synonyms Polyclonal Glyoxalase I antibody, Anti-Glyoxalase I antibody, GLYI antibody, GLO1 antibody, S-D-lactoylglutathione methylglyoxal lyase antibody, GLOD1 antibody, aldoketomutase antibody, lactoylglutathione lyase antibody, glyoxalase domain containing 1 antibody, lactoyl glutathione lyase antibody, methylglyoxalase antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, IHC-P, WB
Immunogen Glyoxalase I antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 184 of Glyoxalase I

Images

Western blot analysis using Glyoxalase I antibody (70R-12932)

Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 12 % SDS PAGE gel and Glyoxalase I antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification Glyoxalase I antibody was purified by antigen-affinity chromatography
Molecular Weight 21 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using Glyoxalase I antibody (70R-12932)</font></div>     | Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 12 % SDS PAGE gel and Glyoxalase I antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Western blot analysis using Glyoxalase I antibody (70R-12932)</font></div>     | Western blot analysis of 30 ug whole cell lysate (A: 293T; B: A431; C: H1299; D: HeLa S3; E: Hep G2; F: MOLT4; G: Raji) using a 12 % SDS PAGE gel and Glyoxalase I antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunofluorescent staining using Glyoxalase I antibody (70R-12932)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using glyoxalase I antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Immunohistochemical staining using Glyoxalase I antibody (70R-12932)</font></div>     | Immunohistochemical staining of paraffin-embedded OVCA using Glyoxalase I antibody at a dilution of 1:100

Availability: In stock

Price: €323.11
Size: 100 ul
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