Western blot analysis using Glyoxalase I antibody (70R-12932)
Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 12 % SDS PAGE gel and Glyoxalase I antibody at a dilution of 1:1000
Glyoxalase I antibody
Western blot analysis using Glyoxalase I antibody (70R-12932)
Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 12 % SDS PAGE gel and Glyoxalase I antibody at a dilution of 1:1000
Western blot analysis using Glyoxalase I antibody (70R-12932)
Western blot analysis of 30 ug whole cell lysate (A: 293T; B: A431; C: H1299; D: HeLa S3; E: Hep G2; F: MOLT4; G: Raji) using a 12 % SDS PAGE gel and Glyoxalase I antibody at a dilution of 1:1000
Immunofluorescent staining using Glyoxalase I antibody (70R-12932)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using glyoxalase I antibody at 1:200 dilution.
Immunohistochemical staining using Glyoxalase I antibody (70R-12932)
Immunohistochemical staining of paraffin-embedded OVCA using Glyoxalase I antibody at a dilution of 1:100
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
Glyoxalase I antibody was purified by antigen-affinity chromatography
Molecular Weight
21 kDa
Form & Buffer
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.