GMCSF protein (30R-2272)
Purified recombinant Human GMCSF protein
Overview
Overview
| Synonyms | Granulocyte/Macrophage-Colony Stimulating Factor protein, GMCSF protein |
|---|---|
| Specificity | Human |
| Species | Human |
| Protein Type | Recombinant |
| Applications | FC |
| Assay Information | A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
Specifications
| Expression System | E.coli |
|---|---|
| Grade & Purity | > 98% pure |
| Molecular Weight | 13 kDa |
| Form & Buffer | Supplied as a sterile liquid with 10 mM sodium phosphate, pH7.2, 150 mM NaCl. 0.22 um filtered. |
| Concentration | 200 ug/ml |
Usage & Assay Information
| Usage Recommendations | FC: = 0.125 ug/test |
|---|---|
| Bioactivity | The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than or equal to 100 pg/ml.This corresponds to a specific activity of greater than or equal to 1 x 10e7 Units/mg. |
Storage & Safety
| Storage | Store at -70 deg C or less. |
|---|---|
| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Human GM-CSF is a differentially glycosylated factor produced mainly by activated T cells and macrophages. Endothelial cells and fibroblasts can also produce GM-CSF after exposure to TNF- alpha, IL-1, IL-2 and IFN-gamma. GM-CSF is found associated with extracellular matrix and in membrane-bound formats too. GM-CSF stimulates proliferation, activation and differentiation of macrophages and granulocytes and their progenitors. |
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