GMCSF protein (30R-2272)

Purified recombinant Human GMCSF protein

Synonyms Granulocyte/Macrophage-Colony Stimulating Factor protein, GMCSF protein
Specificity Human
Species Human
Protein Type Recombinant
Applications FC
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Specifications

Expression System E.coli
Grade & Purity > 98% pure
Molecular Weight 13 kDa
Form & Buffer Supplied as a sterile liquid with 10 mM sodium phosphate, pH7.2, 150 mM NaCl. 0.22 um filtered.
Concentration 200 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.125 ug/test
Bioactivity The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than or equal to 100 pg/ml.This corresponds to a specific activity of greater than or equal to 1 x 10e7 Units/mg.

Storage & Safety

Storage Store at -70 deg C or less.
Endotoxin Levels Less than 0.01 ng/ug cytokine as determined by the LAL assay.

General Information

Biological Significance Human GM-CSF is a differentially glycosylated factor produced mainly by activated T cells and macrophages. Endothelial cells and fibroblasts can also produce GM-CSF after exposure to TNF- alpha, IL-1, IL-2 and IFN-gamma. GM-CSF is found associated with extracellular matrix and in membrane-bound formats too. GM-CSF stimulates proliferation, activation and differentiation of macrophages and granulocytes and their progenitors.

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Availability: In stock

Price: €1,321.06
Size: 100 ug
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