GNAS antibody (70R-12940)

Affinity purified Rabbit polyclonal GNAS antibody

Synonyms Polyclonal GNAS antibody, Anti-GNAS antibody, GSA antibody, adenylate cyclase-stimulating G alpha protein antibody, GPSA antibody, PHP1A antibody, NESP antibody, PHP1B antibody, POH antibody, protein ALEX antibody, GNAS antibody, MGC33735 antibody, GSP antibody, AHO antibody, PHP1C antibody, GNAS1 antibody, guanine nucleotide binding protein (G protein) alpha stimulating activity polypeptide 1 antibody, C20orf45 antibody, guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas antibody
Cross Reactivity Human, Mouse
Applications IHC-P, WB
Immunogen GNAS antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 716 and 998 of GNAS

Images

Western blot analysis using GNAS antibody (70R-12940)

Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10 % SDS PAGE gel and GNAS antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification GNAS antibody was purified by antigen-affinity chromatography
Molecular Weight 111 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.78 mg/ml

Usage & Assay Information

Usage Recommendations IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using GNAS antibody (70R-12940)</font></div>     | Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10 % SDS PAGE gel and GNAS antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using GNAS antibody (70R-12940)</font></div>     | Immunohistochemical staining of paraffin-embedded OVCA using GNAS antibody at a dilution of 1:100
  • <div><font face=Calibri size=2>Western blot analysis using GNAS antibody (70R-12940)</font></div>     | Western blot analysis of 30 ug whole cell lysate (A:A431; B:Hep G2) using a 7.5 % SDS PAGE gel and GNAS antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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