GNPDA1 protein (His tag) (80R-1463)
Purified recombinant Human GNPDA1 protein
|Synonyms||GNPDA1, GPI protein, HLN protein, Glucosamine-6-phosphate isomerase 1 protein, GNPI protein, GNPDA-1, GNPDA 1, GNPDA protein, GNPDA 1 protein, GNPDA-1 protein, Oscillin, GNP1 protein|
Coomassie Blue stained SDS-PAGE of GNPDA1 protein (His tag) (80R-1463)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-289 amino acids: MGSSHHHHHH SSGLVPRGSH MKLIILEHYS QASEWAAKYI RNRIIQFNPG PEKYFTLGLP TGSTPLGCYK KLIEYYKNGD LSFKYVKTFN MDEYVGLPRD HPESYHSFMW NNFFKHIDIH PENTHILDGN AVDLQAECDA FEEKIKAAGG IELFVGGIGP DGHIAFNEPG SSLVSRTRVK TLAMDTILAN ARFFDGELTK VPTMALTVGV GTVMDAREVM ILITGAHKAF ALYKAIEEGV NHMWTVSAFQ QHPRTVFVCD EDATLELKVK TVKYFKGLML VHNKLVDPLY SIKEKETEKS QSSKKPYSD|
|Grade & Purity||> 90% pure|
|Molecular Weight||34.8 kDa (309aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 20% glycerol and 50mM NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Glucosamine-6-phosphate isomerase 1, also known as GNPDA1, catalyzes the conversion of glucosamine-6-phosphate to fructose-6-phosphate, a reaction that under physiological conditions proceeds to the formation of fructose-6-phosphate. It is ubiquitously expressed with highest expression in testes, ovary and heart. In addition, GNPDA1 triggers calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo. Recombinant human GNPDA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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