Goat anti Bovine IgG (FITC) (43C-CB0137)
Goat anti Bovine IgG secondary antibody (FITC)
Overview
Overview
| Synonyms | Goat anti Bovine Immunoglobulin G secondary antibody (FITC) |
|---|---|
| Specificity | Bovine IgG |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, FC, IF |
| Immunogen | Goat anti-bovine IgG (FITC) was raised in goat using bovine IgG F(ab')2 fragment as the immunogen. |
Images
Dot Blot analysis using Goat anti Bovine IgG (FITC) (43C-CB0137)
Dot Blot of Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated. Antigen: Bovine IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Bovine IgG Fab2 Antibody Fluorescein Conjugated at 1:1,000 for 1 HR at RT. Block: MB-070 for 1 HR at RT.
Specifications
| Host | Goat |
|---|---|
| Method of Purification | Goat anti Bovine IgG (FITC) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis. |
| Tag/Conjugate | FITC |
| Form & Buffer | Lyophilized from 0.01M Na3PO4, pH 7.4, with 0.14M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free. |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000 |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. |
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