Goat anti Human IgG (Fab'2) (PE) (43C-CJ0117)
Goat anti Human IgG secondary antibody (Fab'2) (PE)
Overview
Overview
| Synonyms | Goat anti Human Immunoglobulin G secondary antibody (Fab'2) (PE) |
|---|---|
| Specificity | Human IgG (Fab2) |
| Cross Reactivity | To be determined by end-user |
| Applications | FC, IF |
| Immunogen | Goat anti-human IgG (Fab'2) (PE) was raised in goat using human IgG F(c) fragment as the immunogen. |
Images
Western blot analysis using Goat anti Human IgG (Fab'2) (PE) (43C-CJ0117)
Western blot of Phycoerythrin conjugated Goat F(ab’)2 Anti-Human IgG F(c). Lane 1: Human Fc. Lane 2: None. Load: 50 ng per lane. None. Phycoerythrin Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for Human IgG F(c)..
Specifications
| Host | Goat |
|---|---|
| Method of Purification | Goat anti Human IgG (Fab'2) (PE) was purified by affinity chromatography. |
| Tag/Conjugate | PE |
| Form & Buffer | Supplied in liquid form in0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.05% NaN3. Immunoglobulin and protease free. |
Usage & Assay Information
| Usage Recommendations | FC: 1:100-1:250, IF: 1:100-1:250 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The Fab region, (fragment, antigen binding), is the binding region of the antibody. |
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