Goat anti Human IgM (Alk Phos) (43C-CB0999)
Goat anti Human IgM secondary antibody (Alk Phos)
Overview
Overview
| Synonyms | Goat anti Human Immunoglobulin M secondary antibody (Alk Phos) |
|---|---|
| Specificity | Human IgM |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | Goat anti-human IgM (Alk Phos) was raised in goat using human IgM mu mu chain as the immunogen. |
Images
Dot Blot analysis using Goat anti Human IgM (Alk Phos) (43C-CB0999)
Dot Blot of Goat anti-Human IgM (mu chain) Antibody Alkaline Phosphatase Conjugated. Antigen: Human IgM. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Goat anti-Human IgM (mu chain) Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 1 HR at RT. Visualized using NBT-100 Alkaline Phosphatase Substrate for 30 seconds at RT.
Specifications
| Host | Goat |
|---|---|
| Method of Purification | Goat anti Human IgM (Alk Phos) was purified by affinity chromatography. |
| Tag/Conjugate | Alkaline Phosphatase |
| Form & Buffer | 0.05M TRIS-HCl, pH 8.0, with 0.15M NaCl, 0.001M MgCl, 0.0001M ZnCl, 50% glycerol, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free. |
| Concentration | 1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:1,000-1:3,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,000 |
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Storage & Safety
| Storage | Store at 4 deg C. DO NOT FREEZE. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. It is the primary antibody against A and B antigens on red blood cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to antigen. |
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