Goat anti Human IgM (mu chain) (Alk Phos) (43R-1233)
Goat anti Human IgM (mu chain) secondary antibody (Alk Phos)
Overview
Overview
| Synonyms | Goat anti Human Immunoglobulin M (mu chain) secondary antibody (Alk Phos) |
|---|---|
| Specificity | This antibody reacts with heavy chains on human IgM (mu chain). |
| Cross Reactivity | No reactivity is observed to non-immunoglobulin human serum proteins or light chains on all human immunoglobulins. |
| Applications | User optimized |
| Immunogen | Purified Human IgM |
Specifications
| Host | Goat |
|---|---|
| Source | Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. |
| Grade & Purity | > 90% pure |
| Method of Purification | Affinity purified using solid phase Human IgM |
| Tag/Conjugate | Alkaline Phosphatase |
| Form & Buffer | Clear, colorless liquid, containing 30 mM Triethanolamine, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1% (w/v) BSA and 0.05% (w/v) Sodium Azide. Protease/IgG free, pH 7.2. |
| Concentration | 1.50 mg/ml |
Usage & Assay Information
| Usage Recommendations | 1:5000-1:50,000 ELISA/WB, 1:500-1:2000 IHC |
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Storage & Safety
| Storage | Store undiluted liquid at 2-8 deg C. For storage at -20 deg C, aliquot and dilute with an equal volume of glycerol to prevent loss of enzymatic activity. |
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General Information
| Biological Significance | IgM is found in normal plasma at a concentration of 1.2 to 4.0 mg/ml. It is the first immunoglobulin produced during the immune response, the first antibody in neonates, and is involved in pathogenesis of autoimmune diseases. A diminished level of IgM is associated with Wiskott-Aldrich syndrome. |
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