Goat anti Human Kappa Chain (biotin) (43C-CB9125)
Goat anti Human Kappa Chain secondary antibody (biotin)
Overview
Overview
| Synonyms | Goat anti Human Kappa Chain secondary antibody (biotin) |
|---|---|
| Specificity | Human kappa chain |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | Goat anti-human kappa chain (biotin) was raised in goat using human kappa chain as the immunogen. |
Images
Western blot analysis using Goat anti Human Kappa Chain (biotin) (43C-CB9125)
Western blot of Biotin conjugated Human kappa secondary antibody. Lane 1: Human ?. Lane 2: Human ?. Load: 50 ng per lane. None. Biotin rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for Human kappa
Specifications
| Host | Goat |
|---|---|
| Method of Purification | Goat anti Human Kappa Chain (biotin) was purified by affinity chromatography. |
| Tag/Conjugate | Biotin |
| Form & Buffer | Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0 01% NaN3. Immunoglobulin and protease free. |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000 |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). A typical antibody is composed of two immunoglobulin (Ig) heavy chains and two Ig light chains. |
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