Goat anti Mouse IgG (Fab'2) (PE) (43C-CJ0218)
Goat anti Mouse IgG secondary antibody (Fab'2) (PE)
Overview
Overview
| Synonyms | Goat anti Mouse Immunoglobulin G secondary antibody (Fab'2) (PE) |
|---|---|
| Specificity | Murine IgG (Fab2) |
| Cross Reactivity | To be determined by end-user |
| Applications | FC, IF |
| Immunogen | Goat anti-mouse IgG (Fab'2) (PE) was raised in goat using murine IgG F(ab’)2 fragment as the immunogen. |
Images
Western blot analysis using Goat anti Mouse IgG (Fab'2) (PE) (43C-CJ0218)
Western blot of Phycoerythrin conjugated Goat F(ab’)2 Anti-Mouse IgG F(ab’)2 . Lane 1: Mouse Fab2. Lane 2: None. Load: 50 ng per lane. None. Phycoerythrin Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 kDa, 25 kDa for Mouse IgG F(ab’)2..
Specifications
| Host | Goat |
|---|---|
| Method of Purification | Goat anti Mouse IgG (Fab'2) (PE) was purified by immunoaffinity chromatography, pepsin digestion and chromatographic separation. |
| Tag/Conjugate | PE |
| Form & Buffer | 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free. |
| Concentration | 0.5 mg/ml |
Usage & Assay Information
| Usage Recommendations | FC: 1:100-1:250, IF: 1:100-1:250 |
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Storage & Safety
| Storage | Store at 4 deg C. DO NOT FREEZE. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The Fab region, (fragment, antigen binding), is the binding region of the antibody. |
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