Goat anti Mouse IgG (FITC) (43R-1359)
Goat anti Mouse IgG secondary antibody (FITC)
Overview
Overview
| Synonyms | Goat anti Mouse Immunoglobulin G secondary antibody (FITC) |
|---|---|
| Specificity | This antibody reacts with heavy (gamma) chains on mouse IgG. |
| Cross Reactivity | No reactivity is observed to non-immunoglobulin human serum proteins or light chains on all mouse immunoglobulins or F(ab)’2 fragments of mouse IgG. |
| Applications | FC, IF, IHC |
| Immunogen | Purified Mouse IgG |
Specifications
| Host | Goat |
|---|---|
| Source | Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. |
| Grade & Purity | > 95% pure |
| Method of Purification | Affinity purified using solid phase Mouse IgG (H&L) |
| Tag/Conjugate | FITC |
| Form & Buffer | Clear, fluorescent yellow liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) Sodium Azide. Protease/IgG free, pH 7.2. |
| Concentration | 1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications |
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Storage & Safety
| Storage | Store at 2-8 deg C. |
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General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG constitutes 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells. |
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