Goat anti Mouse IgG2a (HRP) (43R-IG064hrp)
Goat anti Mouse IgG2a secondary antibody (HRP)
Overview
Overview
| Synonyms | Mouse Immunoglobulin G2 alpha antibody (HRP), Mouse IgG2a antibody (HRP) |
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| Specificity | Murine |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, ICC, IHC, WB |
Specifications
| Host | Goat |
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| Isotype | IgG |
| Method of Purification | Goat anti Mouse IgG2a (HRP) was purified by affinity chromatography. |
| Tag/Conjugate | HRP |
| Form & Buffer | 50mM HEPES pH 7.1, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2 with 0.2% BSA and 0.09% NaN3. |
| Concentration | 1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:10,000-1:100,000, ICC: 1:200-1:500, IHC: 1:200-1:500, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000 |
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Storage & Safety
| Storage | Store at 4 deg C. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | IgG2 is the only IgG subclass which passes through the placenta at a level generally lower than that found in the mother. A deficiency of IgG2 indicates a poor antibody response to bacterial polysaccharides and can lead to increased susceptibility to infections caused by encapsulated bacteria. |
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