Goat anti Rabbit IgG Fc (41C-CB1136)
Goat anti Rabbit IgG secondary antibody
Overview
Overview
| Synonyms | Goat anti Rabbit Immunoglobulin G secondary antibody |
|---|---|
| Specificity | Rabbit IgG |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | Goat anti-rabbit IgG was raised in goat using rabbit IgG F(c) fragment as the immunogen. |
Images
Western blot analysis using Goat anti Rabbit IgG Fc (41C-CB1136)
Western Blot of Rabbit F(c) secondary antibody. Lane 1: Rabbit F(c). Lane 2: None. Load: 50 ng per lane. Rabbit F(c) antibody at 1:1,000 overnight at 4 deg C. DyLight649 Goat secondary antibody at 1:20,000 for 30 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 kDa, 25 kDa for Rabbit F(c)..
Specifications
| Host | Goat |
|---|---|
| Method of Purification | Goat anti Rabbit IgG was purified by affinity chromatography. |
| Form & Buffer | 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. |
| Concentration | 2.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. |
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