Goat anti Rat IgG (Fab'2) (41C-CJ0317)
Goat anti Rat IgG secondary antibody (Fab'2)
Overview
Overview
| Synonyms | Goat anti Rat Immunoglobulin G secondary antibody (Fab'2) |
|---|---|
| Specificity | Rat IgG (Fab2) |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | Goat anti-rat IgG (Fab'2) was raised in goat using rat IgG F(ab')2 fragment as the immunogen. |
Specifications
| Host | Goat |
|---|---|
| Method of Purification | Goat anti Rat IgG (Fab'2) was purified by delipidation, salt fractionation and ion exchange chromatography. |
| Form & Buffer | 0.01M Na3PO4, pH 7.6, with 0.25M NaCl. |
| Concentration | 1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The Fab region, (fragment, antigen binding), is the binding region of the antibody. |
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