GOT1 protein (80R-4223)

Recombinant Mouse GOT1 protein with His tag

Synonyms Mouse GOT1 protein, Recombinant GOT1 protein, Glutamate oxaloacetate transaminase 1 soluble protein, cAspAT protein
Species Mouse
Protein Type Recombinant
Applications Control, SDS-PAGE

Images

Coomassie blue stained SDS-PAGE showing analysis of GOT1 protein(80R-4223)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-413 amino acids of Mouse GOT1 protein: MGSSHHHHHH SSGLVPRGSH MGSMAPPSVF AQVPQAPPVL VFKLTADFRD DPDPRKVNLG VGAYRTDESQ PWVLPVVRKV EQKIANDNSL NHEYLPILGL AEFRSCASRL VLGDNSPAIR ENRVGGVQSL GGTGALRIGA DFLGRWYNGT DNKNTPIYVS SPTWENHNAV FSAAGFKDIR PYCYWDAEKR GLDLQGFLND LENAPEFSIF VLHACAHNPT GTDPTPEQWK QIAAVMQRRF LFPFFDSAYQ GFASGDLEKD AWAIRYFVSE GFELFCAQSF SKNFGLYNER VGNLTVVGKE SDSVLRVLSQ MEKIVRITWS NPPAQGARIV AATLSDPELF KEWKGNVKTM ADRILTMRSE LRARLEALKT PGTWSHITEQ IGMFSFTGLN PKQVEYLVNE KHIYLLPSGR INMCGLTTKN LDYVATSIHE AVTKIQ
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 48.6 kDa
Tag/Conjugate His tag
Form & Buffer Supplied as liquid in PBS (pH 7.4) containing 10% glycerol and 1mM DTT
Concentration 500 ug/ml

Storage & Safety

Storage Store at 4 deg C for short term. For long term storage, aliquot and store at -20 deg C. Avoid repeated freeze thaw cycles.

General Information

Biological Significance Got1 also known as Glutamate oxaloacetate transaminase 1 is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT1 plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant mouse Got1 fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • 

Coomassie blue stained SDS-PAGE showing analysis of GOT1 protein(80R-4223)



 | 

Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €241.31
Size: 100 ug
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