GOT2 protein (80R-4288)

Purified Recombinant GOT2 protein (His tagged)

Synonyms KAT4 protein, KATIV protein, mitAAT protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis using GOT2 protein (His tag) (80R-4288)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 30-430 aa: MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 47 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl
Concentration 500 ug/ml

Usage & Assay Information

Bioactivity Specific activity > 60 units/mg

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeat freeze/thaw cycles
Endotoxin Levels < 1.0 Eu per 1 microgram of protein

General Information

Biological Significance GOT2, also known as glutamate oxaloacetate transaminase 2, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. glutamate oxaloacetate transaminas is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

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SDS-PAGE analysis using GOT2 protein (His tag) (80R-4288)



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Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €126.79
Size: 20 ug
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