GPI antibody (70R-10234)
Affinity purified rabbit polyclonal GPI antibody
Overview
Overview
| Synonyms | Polyclonal GPI antibody, Anti-GPI antibody, NLK antibody, PGI antibody, glucose-6-phosphate isomerase antibody, PHI antibody, SA36 antibody, AMF antibody, GNPI antibody |
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| Cross Reactivity | Human |
| Applications | WB |
| Immunogen | GPI antibody was raised in rabbit using the C terminal of GPI as the immunogen |
| Assay Information | GPI Blocking Peptide, catalog no. 33R-2268, is also available for use as a blocking control in assays to test for specificity of this GPI antibody |
Images
Western Blot analysis using GPI antibody (70R-10234)
Western Blot showing GPI antibody used at a concentration of 1 ug/ml against HepG2 Cell Lysate
Specifications
| Host | Rabbit |
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| Form & Buffer | Lyophilized powder. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer. |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Usage Recommendations | Optimal conditions to be determined by the end user |
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Storage & Safety
| Storage | Store at 4 deg C, following reconstitution, aliquot and store at -20 deg C. Avoid repeated freeze-thaw cycles. |
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General Information
| Biological Significance | This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. |
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