GPR18 antibody (70R-49807)

Purified Polyclonal GPR18 antibody

Synonyms Polyclonal GPR18 antibody, Anti-GPR18 antibody, GPCRW antibody, N-arachidonyl glycine receptor antibody, NAGly receptor antibody, G-protein coupled receptor 18 antibody
Specificity Recognizes endogenous levels of GPR18 protein
Cross Reactivity Mouse, Rat
Applications FC, ICC, IF, WB
Immunogen GPR18 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR18 as the immunogen

Images

Western blot analysis using GPR18 antibody (70R-49807)

Western blot analysis of GPR18 expression in HEK293T (A); Jurkat (B); mouse brain (C); rat brain (D) whole cell lysates.

Specifications

Host Rabbit
Method of Purification GPR18 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IF: 1:100 - 1:500, ICC: 1:100 - 1:500, FC: 1:100 - 1:400

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance GPR18 is a receptor for N-arachidonyl glycine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. May contribute to regulation of the immune system.

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Images

  • 

Western blot analysis using GPR18 antibody (70R-49807)



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Western blot analysis of GPR18 expression in HEK293T (A); Jurkat (B); mouse brain (C); rat brain (D) whole cell lysates.
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Immunofluorescent analysis using GPR18 antibody (70R-49807)



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Immunofluorescent analysis of GPR18 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Availability: In stock

Price: €197.96
Size: 100 ul
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