GPT2 protein (80R-4285)
Purified Recombinant GPT2 protein (His tagged)
Overview
Overview
| Synonyms | AAT2 protein, ALT2 protein |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
SDS-PAGE analysis using GPT2 protein (His tag) (80R-4285)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-523 aa: MGSSHHHHHH SSGLVPRGSH MGSMQRAAAL VRRGCGPRTP SSWGRSQSSA AAEASAVLKV RPERSRRERI LTLESMNPQV KAVEYAVRGP IVLKAGEIEL ELQRGIKKPF TEVIRANIGD AQAMGQQPIT FLRQVMALCT YPNLLDSPSF PEDAKKRARR ILQACGGNSL GSYSASQGVN CIREDVAAYI TRRDGGVPAD PDNIYLTTGA SDGISTILKI LVSGGGKSRT GVMIPIPQYP LYSAVISELD AIQVNYYLDE ENCWALNVNE LRRAVQEAKD HCDPKVLCII NPGNPTGQVQ SRKCIEDVIH FAWEEKLFLL ADEVYQDNVY SPDCRFHSFK KVLYEMGPEY SSNVELASFH STSKGYMGEC GYRGGYMEVI NLHPEIKGQL VKLLSVRLCP PVSGQAAMDI VVNPPVAGEE SFEQFSREKE SVLGNLAKKA KLTEDLFNQV PGIHCNPLQG AMYAFPRIFI PAKAVEAAQA HQMAPDMFYC MKLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPVEKLKTV LQKVKDFHIN FLEKYA |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 90% pure |
| Molecular Weight | 60.3 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in liquid form in 20 mM Tris-HCl buffer (pH7.5) containing 30% glycerol, 2mM DTT, 0.2M NaCl |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Bioactivity | Specific activity > 100units/mg |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeat freeze/thaw cycles |
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General Information
| Biological Significance | Alanine aminotransferase 2, also known as GPT2, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. GPT2 expression is high in muscle, fat and kidney. Recombinant human GPT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
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