Granulocytes antibody (10R-6423)

Mouse monoclonal Granulocytes antibody

Synonyms Monoclonal Granulocytes antibody, Anti-Granulocytes antibody
Specificity Human
Applications FC, Functional Assay, IHC, IP
Assay Information A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest

Images

Flow Cytometric analysis using Granulocytes antibody (10R-6423)

Staining of rat splenocytes with Anti-Rat Granulocyte Marker (FITC) (filled histogram). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.

Specifications

Host Mouse
Clone HIS48
Isotype IgM
Method of Purification Granulocytes antibody was purified by affinity chromatography.
Form & Buffer Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations FC: = 0.25 ug/test

Storage & Safety

Storage Store at 2-8 deg C. Do not freeze. Protect from light.

General Information

Biological Significance The HIS48 monoclonal antibody reacts with a molecule expressed on the surface of all rat granulocytes. The antigen recognized by HIS48 is also expressed by erythroid lineage cells during their developmental stages in the bone marrow. HIS48 is commonly used as a phenotypic marker for rat granulocytes.

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Images

  • Flow Cytometric analysis using Granulocytes antibody (10R-6423) | Staining of rat splenocytes with Anti-Rat Granulocyte Marker (FITC) (filled histogram). Appropriate Isotype Controls were used (open histogram). Total viable cells were used for analysis.

Availability: In stock

Price: €192.23
Size: 100 ug
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