Granzyme B protein (30R-2408)

Purified recombinant Human Granzyme B protein

Synonyms Cathepsin G-like 1 protein, Lymphocyte protease protein, SECT protein, C11 protein, Granzyme-2 protein, CTSGL1 protein, CTLA-1 protein, Fragmentin-2 protein, Human lymphocyte protein, T-cell serine protease 1-3E protein, Cytotoxic T-lymphocyte proteinase 2 protein, HLP protein, GranzymeB protein
Species Human
Protein Type Recombinant

Images

Western blot analysis using Granzyme B protein (30R-2408)

Specifications

Expression System Insect cells
Method of Purification Granzyme B protein was purified using a a proprietary chromatographic technique.
Molecular Weight 25.5 kDa
Form & Buffer Lyophilized from 20 mM HEPES buffer pH 7.5. Reconstitute5 ugin 50 ul sterilewater togive afinal concentrationof 0.1 ug/ul.

Usage & Assay Information

Bioactivity Activity of Granzyme B protein was tested using the synthetic peptide, IETD-AFC, as a substrate. Granzyme B protein releases AFC and activity can be quantified by fluorescent detection of free AFC at Ex/Em=380/500 nm.

Storage & Safety

Storage Store aliquots at -20 deg C. Avoid repeated freeze thaw.

General Information

Biological Significance Granzyme B is a serine protease stored in granules of activated cytotoxic T cells and NK cells. Upon target cell contact, granzyme B is directionally exocytosed and assisted by perforin, enters the target cell. With its unique substrate specificity, granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.

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Images

  • Western blot analysis using Granzyme B protein (30R-2408)

Availability: In stock

Price: €364.01
Size: 5 ug
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