GRK2 antibody (70R-49347)

Purified Polyclonal GRK2 antibody

Synonyms Polyclonal GRK2 antibody, Anti-GRK2 antibody, BARK antibody, BARK1 antibody, GRK2 antibody, Beta-adrenergic receptor kinase 1 antibody, Beta-ARK-1 antibody, G-protein coupled receptor kinase 2 antibody
Specificity Recognizes endogenous levels of GRK2 protein
Cross Reactivity Mouse, Rat, Bovine
Applications IHC, WB
Immunogen GRK2 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GRK2 as the immunogen

Images

Western blot analysis using GRK2 antibody (70R-49347)

Western blot analysis of GRK2 (pS29) expression in Ramos (A); THP1 (B); U937 (C); C6 (D) whole cell lysates.

Specifications

Host Rabbit
Method of Purification GRK2 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance GRK2 phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors.

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Images

  • 

Western blot analysis using GRK2 antibody (70R-49347)



 | 

Western blot analysis of GRK2 (pS29) expression in Ramos (A); THP1 (B); U937 (C); C6 (D) whole cell lysates.
  • 

Immunohistochemical analysis using GRK2 antibody (70R-49347)



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Immunohistochemical analysis of GRK2 (pS29) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €233.95
Size: 100 ul
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