GRK2 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GRK2 as the immunogen
Western blot analysis using GRK2 antibody (70R-49348)
Western blot analysis of GRK2 expression in HeLa (A); HEK293T (B); NIH3T3 (C); H9C2 (D) whole cell lysates.
GRK2 antibody
Western blot analysis using GRK2 antibody (70R-49348)
Western blot analysis of GRK2 expression in HeLa (A); HEK293T (B); NIH3T3 (C); H9C2 (D) whole cell lysates.
Immunofluorescent analysis using GRK2 antibody (70R-49348)
Immunofluorescent analysis of GRK2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Specifications
Host
Rabbit
Method of Purification
GRK2 antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide