GRK2 antibody (70R-49348)

Purified Polyclonal GRK2 antibody

Synonyms Polyclonal GRK2 antibody, Anti-GRK2 antibody, BARK antibody, BARK1 antibody, GRK2 antibody, Beta-adrenergic receptor kinase 1 antibody, Beta-ARK-1 antibody, G-protein coupled receptor kinase 2 antibody
Specificity Recognizes endogenous levels of GRK2 protein
Cross Reactivity Mouse, Rat, Bovine, Dog, Pig
Applications ICC, IF, IHC, WB
Immunogen GRK2 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GRK2 as the immunogen

Images

Western blot analysis using GRK2 antibody (70R-49348)

Western blot analysis of GRK2 expression in HeLa (A); HEK293T (B); NIH3T3 (C); H9C2 (D) whole cell lysates.

Specifications

Host Rabbit
Method of Purification GRK2 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance GRK2 is a ubiquitous protein kinase of the GRK family.

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Images

  • 

Western blot analysis using GRK2 antibody (70R-49348)



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Western blot analysis of GRK2 expression in HeLa (A); HEK293T (B); NIH3T3 (C); H9C2 (D) whole cell lysates.
  • 

Immunofluorescent analysis using GRK2 antibody (70R-49348)



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Immunofluorescent analysis of GRK2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Availability: In stock

Price: €197.96
Size: 100 ul
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