grxB protein (His tag) (80R-1797)

Purified recombinant E.coli grxB protein

Synonyms bA101E13.1 protein, GRLX2 protein, Grx2 protein, glutaredoxin 2 protein, CGI-133 protein
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of grxB protein (His tag) (80R-1797)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-215 amino acids: MGSSHHHHHH SSGLVPRGSH MKLYIYDHCP YCLKARMIFG LKNIPVELHV LLNDDAETPT RMVGQKQVPI LQKDDSRYMP ESMDIVHYVD KLDGKPLLTG KRSPAIEEWL RKVNGYANKL LLPRFAKSAF DEFSTPAARK YFVDKKEASA GNFADLLAHS DGLIKNISDD LRALDKLIVK PNAVNGELSE DDIQLFPLLR NLTLVAGINW PSRVADYRDN MAKQTQINLL SSMAI
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 26.5 kDa (235aa) confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 10% glycerol and 50mM NaCl.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance GrxB (Glutaredoxin-2) belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes of approximately one hundred amino-acid residues that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. This protein is involved in reducing some disulfides in a coupled system with glutathione reductase. It does not act as hydrogen donor for ribonucleotide reductase. Recombinant E. coli grxB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of grxB protein (His tag) (80R-1797) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €277.55
Size: 100 ug
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