GST protein (His tag) (80R-1204)
Purified recombinant Schistosoma japonicum GST protein
Overview
Overview
| Synonyms | Sj26 protein protein, Glutathione S-Transferase class-mu 26 kDa isozyme Glutathione S Transferase., SjGST protein |
|---|---|
| Species | Schistosoma japonicum |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
| Assay Information | 1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2. Equilibrate to 25 DEG C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 50ul of recombinant GST protein with various concentrations (1ug, 2ug, 5ug) in 950ul reaction buffer. 4. Mix by inversion and record the increase at A340nm for 5 minutes. |
Images
Coomassie Blue stained SDS-PAGE of GST protein (His tag) (80R-1204)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-224 amino acids: MGSSHHHHHH SSGLVPRGSH MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 28.3 kDa (244aa), confirmed by MALDI-TOF. |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as a liquid in PBS, pH 7.4, containing 10% glycerol. |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Bioactivity | Specific activity is 2.8-3.3 units/mg, and is defined as the amount of enzyme that conjugate 1.0 uM of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 DEG C. |
|---|
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
|---|
General Information
| Biological Significance | Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form a S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E. coli source. Recombinant human GST, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
|---|
Reviews
You need to be logged in to write a review. Please login here
Sorry there are currently no reviews for this product