GSTA4 protein (80R-4286)
Purified Recombinant GSTA4 protein (His tagged)
Overview
Overview
| Synonyms | GSTA4-4 protein, GTA4 protein |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
SDS-PAGE analysis using GSTA4 protein (His tag) (80R-4286)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-222 aa: MGSSHHHHHH SSGLVPRGSH MGSHMAARPK LHYPNGRGRM ESVRWVLAAA GVEFDEEFLE TKEQLYKLQD GNHLLFQQVP MVEIDGMKLV QTRSILHYIA DKHNLFGKNL KERTLIDMYV EGTLDLLELL IMHPFLKPDD QQKEVVNMAQ KAIIRYFPVF EKILRGHGQS FLVGNQLSLA DVILLQTILA LEEKIPNILS AFPFLQEYTV KLSNIPTIKR FLEPGSKKKP PPDEIYVRTV YNIFRP |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 28.3 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in liquid form in 20 mM Tris-HCl buffer (pH 8.0), 2mM DTT, 20% glycerol,100 mM NaCl |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Bioactivity | Specific activity > 4,000 pmol/min/ug |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeat freeze/thaw cycles |
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General Information
| Biological Significance | GSTA4, also known as glutathione S-transferase A4, belongs to the GST superfamily. This enzyme is involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. GSTA4 shows very high activity with reactive carbonyl compounds such as alk-2-enals. GSTA4 is highly effective in catalyzing the conjugate addition of reduced glutathione to 4-hydroxynonenal, an important product of peroxidative degradation of arachidonic acid and a commonly used biomarker for oxidative damage in tissue. This enzyme is expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver. Recombinant human GSTA4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
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