GSTM1 protein (30R-1261)

Purified recombinant Mouse GSTM1 protein

Synonyms GST class-mu1 protein, GSTM-1, GST class mu 1 protein, GSTM1, GSTM 1 protein, GSTM1., GST1 protein, GSTM-1 protein, GST class Mu protein, pmGT10 protein, Glutathione S-transferase Mu 1 Glutathione S transferase mu protein, GSTM 1, GST mu protein, GST 1-1 protein
Species Mouse
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2. Equilibrate to 25 DEG C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 50ul of recombinant mouse GSTM1 protein with various concentrations (1ug, 2ug, 5ug) in 950ul reaction buffer. 4. Mix by inversion and record the increase at A340nm for 5 minutes.

Images

Coomassie Blue stained SDS-PAGE of GSTM1 protein (30R-1261)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-218 amino acids: MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 25.9 kDa (218 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
Form & Buffer Supplied as a liquid in PBS, pH 7.4, containing 5mM glutathione.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is 3 units/mg, and is defined as the amount of enzyme that conjugate 1.0 uM of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 DEG C.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance GSTM1 is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant GSTM1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of GSTM1 protein (30R-1261) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €204.50
Size: 100 ug
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