GSTT1 protein (His tag) (80R-1167)
Purified recombinant Human GSTT1 protein
|Synonyms||GSTT-1, Glutathione transferase T1-1 protein, GST class-theta-1 protein, Glutathione S transferase theta 1 protein, GSTT 1, Glutathione S-transferase theta 1 EC 188.8.131.52 protein, Glutathione S-transferase theta 1 protein, GSTT 1 protein, GST 5 5 protein, GSTT-1 protein, GSTT1 protein, GST class theta 1 protein, GST CL1 protein, Glutathione S transferase 5 protein, GSTT1, Glutathione transferase T1 1 protein,|
Coomassie Blue stained SDS-PAGE of GSTT1 protein (His tag) (80R-1167)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-240 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMGL ELYLDLLSQP CRAVYIFAKK NDIPFELRIV DLIKGQHLSD ACAQVNPLKK VPALKDGDFT LTESVAILLY LTRKYKVPDY WYPQDLQARA RVDEYLAWQH TTLRRSCLRA LWHKVMFPVF LGEPVSPQTL AATLAELDVT LQLLEDKFLQ NKAFLTGPHI SLADLVAITE LMHPVGAGCQ VFEGRPKLAT WRQRVEAAVG EDLFQEAHEV ILKAKDFPPA DPTIKQKLMP WVLAMIR|
|Grade & Purity||> 95% pure|
|Molecular Weight||31.5 kDa (277aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Glutathione S-transferase theta 1 (GSTT1) is one of the GSTs’ four main classes: alpha, mu, pi, theta. This enzyme is involved in activation and detoxification reactions and catalyzes the conjugation of industrial chemicals, e.g. epoxybutane, ethylene oxides, halomethane with glutathione. The absence of the GSTTI enzyme, might determine the individual risk for development of acquired aplastic anemia and acute myeloid leukemia. Recombinant human GSTT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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