H3K27me3 antibody (20R-1013)

Rabbit monoclonal H3K27me3 antibody

Synonyms Monoclonal H3K27me3 antibody, Anti H3K27me3 antibody, H3K27me3, HK27me 3,HK27me-3,HK27me 3 antibody, HK27me-3 antibody
Cross Reactivity To be determined by end-user
Applications Dot Blot, IF, WB
Immunogen H3K27me3 antibody was raised in rabbit using a synthetic Histone H3 peptide with Lys27 tri-methylated and conjugated to KLH as the immunogen

Images

Immunofluorescent staining of H3K27me3 antibody (20R-1013)

Immunofluorescence analysis of H3K27me3 in 293T cells. Green: anti-H3K27me3; Blue: DAPI. Primary antibody 1:500.

Specifications

Host Rabbit
Isotype Rabbit IgG
Form & Buffer Supplied in PBS, with 0.005% NaN3, PH 7.4
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations Dot blot: 1:500, IF: 1:100-1:500, WB: 1:1,000-1:4,000

Storage & Safety

Storage Store at -20 deg C, stable for one year at this temperature

General Information

Biological Significance Histones are highly alkaline proteins found in eukaryotic cell nuclei that package and order the DNA into structural units called nucleosomes. They are the chief protein components of chromatin, acting as spools around which DNA winds, and play a role in gene regulation. Without histones, the unwound DNA in chromosomes would be very long (a length to width ratio of more than 10 million to one in human DNA). For example, each human cell has about 1.8 meters of DNA, but wound on the histones it has about 90 micrometers (0.09 mm) of chromatin, which, when duplicated and condensed during mitosis, result in about 120 micrometers of chromosomes.

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Images

  • Immunofluorescent staining of H3K27me3 antibody (20R-1013) | Immunofluorescence analysis of H3K27me3 in 293T cells. Green: anti-H3K27me3; Blue: DAPI. Primary antibody 1:500.
  • Western Blot analysis using H3K27me3 antibody (20R-1013) | Western Blot analysis of endogenous H3K27me3 detected in 293T nuclear extract. Primary antibody 1:1000.

Availability: In stock

Price: €319.02
Size: 100 ul
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