HA Tag antibody (10R-2935)
Mouse monoclonal HA antibody
Overview
Overview
| Synonyms | Monoclonal HA Tag antibody, Anti-HA Tag antibody, HA antibody, Hemagglutinin antibody, HA epitope antibody |
|---|---|
| Specificity | HA tag |
| Applications | Dot Blot, ELISA, IP, WB |
| Immunogen | HA antibody was raised in mouse using YPYDVPDYA synthetic peptide conjugated to KLH as the immunogen. |
Images
Western Blot analysis using HA Tag antibody (10R-2935)
1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with HA-tagged protein vector; unstransfected (1) and transfected (2).
Specifications
| Host | Mouse |
|---|---|
| Clone | HA.C5 |
| Isotype | IgG3 |
| Method of Purification | HA antibody was purified by Protein A affinity chromatography. |
| Form & Buffer | Supplied as a liquid in 10mM PBS, pH 7.2, with 0.05% NaN3 |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Usage Recommendations | WB: 1:1000-3000 |
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Storage & Safety
| Storage | Store at 4 deg C for several days to weeks. Add 0.1% NaN3 and store at -20 deg C for longer periods. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | The HA (human influenza hemagglutinin) epitope tag is frequently engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized HA epitope represents the amino acid sequence YPYDVPDYA |
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