HAMA blocking reagent (85R-1014)
HAMA blocking reagent for use in assays specific for clinical false positive samples
Overview
Overview
| Synonyms | HAMA blocking reagent, Heterophilic Immunoglobulin Blocking Reagent |
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| Applications | ELISA, IF, IHC, Immunoassays, Rapid Test |
| Assay Information | Can be optimized for most assay types |
Specifications
| Form & Buffer | 10mM PBS, 50% glycerine pH 7.4. |
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Usage & Assay Information
| Usage Recommendations | Use at 1:100-1:200; Recommended for application to immunoassays to eliminate false positives in clinical samples |
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Storage & Safety
| Storage | Store at 2-8 deg C for short term storage, store at -20 deg C for long term. |
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General Information
| Biological Significance | Heterophilic Antibody (HA) interference in double-antibody sandwich reaction models can lead to false positive results due to non specific binding to capture and detection reagents. In particular, non specific binding of Human Anti-Mouse Antibody (HAMA) occurs in approximately 10% of immunodiagnostic assays. Other types of HA include, Heterophilic Antibody Goat Antibody (HAGA), Heterophilic Antibody Sheep Antibody (HASA), and Heterophilic Antibody Rabbit Antibody (HARA). This blocking reagent binds to the interferent HAMAs blocking its reaction with capture antibody or conjugated antibody. |
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