HCVE2 protein (His tag) (80R-3891)
Purified recombinant HCVE2 protein (His tag)
Overview
Overview
| Synonyms | E2 protein |
|---|---|
| Species | Viral |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
SDS Page of HCVE2 protein (His tag) (80R-3891)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 482-671 aa: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSERPY CWHYPPRPCG IVPAKSVCGP VYCFTPSPVV VGTTDRSGAP TYSWGANDTD VFVLNNTRPP LGNWFGCTWM NSTGFTKVCG APPCVIGGVG NNTLLCPTDC FRKHPEATYS RCGSGPWITP RCMVDYPYRL WHYPCTINYT IFKVRMYVGG VEHRLEAACN WTRGERCDLE DRDRSELSPL LLSTTQ |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 80% pure |
| Method of Purification | HCVE2 protein was purified by proprietary chromatographic techniques |
| Molecular Weight | 25.4 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol |
| Concentration | 250 ug/ml |
Storage & Safety
| Storage | Can be stored at 4 deg C short term. For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles |
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General Information
| Biological Significance | E1 and E2 glycoproteins form a heterodimer that is involved in virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. E1/E2 heterodimer binds to human LDLR, CD81 and SCARB1/SR-BI receptors, but this binding is not sufficient for infection, some additional liver specific cofactors may be needed. The fusion function may possibly be carried by E1. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. |
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