HDHD2 protein (His tag) (80R-1579)
Purified recombinant Human HDHD2 protein
|Synonyms||HDHD-2 protein, HDHD2, DKFZp564D1378, HDHD 2, Haloacid dehalogenase-like hydrolase domain containing 2 protein, 3110052N05Rik protein, HDHD 2 protein, HDHD-2|
Coomassie Blue stained SDS-PAGE of HDHD2 protein (His tag) (80R-1579)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-259 amino acids: MGSSHHHHHH SSGLVPRGSH MAACRALKAV LVDLSGTLHI EDAAVPGAQE ALKRLRGASV IIRFVTNTTK ESKQDLLERL RKLEFDISED EIFTSLTAAR SLLERKQVRP MLLVDDRALP DFKGIQTSDP NAVVMGLAPE HFHYQILNQA FRLLLDGAPL IAIHKARYYK RKDGLALGPG PFVTALEYAT DTKATVVGKP EKTFFLEALR GTGCEPEEAV MIGDDCRDDV GGAQDVGMLG ILVKTGKYRA SDEEKINPPP YLTCESFPHA VDHILQHLL|
|Grade & Purity||> 95% pure|
|Molecular Weight||30.6 kDa (279aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 10% glycerol and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Haloacid dehalogenase-like hydrolase domain containing 2, also known HDHD2, belongs to the HAD-like hydrolase superfamily. This family of hydrolase enzymes includes L-2-haloacid dehalogenase, epoxide hydrolases and phosphatases. HDHD2 has two active sites, an L-2-haloacid dehalogenase and a carboxylate group. The L-2-haloacid dehalogenase active site catalyzes the hydrolytic dehalogenation of D- and L-2-haloalkanoic acids, producing L- and D-2-hydroxyalkanoic acids. Recombinant human HDHD2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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