Heparan Sulphate Proteoglycan antibody (10R-8070)
Rat monoclonal Heparan Sulphate Proteoglycan antibody
Overview
Overview
| Synonyms | Monoclonal Heparan Sulphate Proteoglycan antibody, Anti-Heparan Sulphate Proteoglycan antibody, Heparan Sulphate Proteoglycan, HSPG antibody, HSPG |
|---|---|
| Specificity | Recognizes domain IV of the core protein of the large heparan sulphate proteoglycan or perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to heparitinase treatment |
| Cross Reactivity | Bovine, Human, Mouse, Rat |
| Applications | IHC, IHC-F, IHC-P, IP, WB |
| Immunogen | Heparan Sulphate Proteoglycan antibody was raised in Rat using high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG as the immunogen. |
Specifications
| Host | Rat |
|---|---|
| Clone | A7L6 |
| Isotype | IgG2a |
| Form & Buffer | Supplied in PBS containing 0.09% sodium azide |
Usage & Assay Information
| Usage Recommendations | IHC: 1:25-1:200, WB: 1:100-1:1000 |
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General Information
| Biological Significance | Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors. |
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