Hepatitis C Virus NS5 Genotype 2a protein (30R-AH010)

Hepatitis C Virus NS5 Genotype-2a recombinant protein

Synonyms Hepatitis C protein, HCV protein, HCV Recombinant protein, Hep C protein, HCV NS5 Genotype 2a protein
Species Viral
Protein Type Recombinant
Applications ELISA, WB

Specifications

Residues The E.coli derived recombinant protein contains the HCV NS5 Genotype 2a immunodominant regions, amino acids 2212-2313. The protein is fused to a GST tag at N-terminus.
Expression System E.coli
Grade & Purity > 95% pure
Method of Purification Hepatitis C Virus NS5 Genotype 2a protein was purified by a proprietary chromatographic technique.
Tag/Conjugate GST tag
Form & Buffer 50mM Tris, pH 8.0 and 5mM EDTA.

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance HCV is a small 50nm, enveloped, single-stranded, positive sense RNAvirus in the family Flaviviridae. HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host's immune response. Hepatitis C virus is classified into six genotypes(1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2, 3, 5 and 6).

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