HGF protein (30R-AH020)
Purified recombinant Human HGF protein
Overview
Overview
| Synonyms | SF protein, Scatter Factor protein, Hepatopoietin protein, Hepatocyte Growth Factor protein, HPTA protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | User optimized |
Specifications
| Expression System | Hi-5 cells |
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| Grade & Purity | > 98% pure |
| Form & Buffer | Lyophilized from 0.1M L-Arginine HCL, with 10mM tris, pH8.0 and 200mM NaCl. Reconstitute in water to a concentration of 0.5 mg/ml. |
Usage & Assay Information
| Bioactivity | The ED50 was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0-40.0 ng/ml. |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Endotoxin Levels | Endotoxin level is less than 0.1 ng per ug (1 EU/ug). |
General Information
| Biological Significance | HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and Mouse HGF are cross-reactive. Human HGF is expressed as a linear 697 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains (a-chain and beta-chain) held by a single disulfide bond resulting in formation of a biologically active heterodimer. |
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