HHV8 antibody (10R-1142)

Mouse monoclonal HHV8 antibody

Synonyms Monoclonal HHV8 antibody, Anti-HHV8 antibody, HHV -8 antibody, HHV -8, HHV 8, Kaposi's sarcoma-associated herpesvirus antibody, HHV 8 antibody, HHV8, Human herpesvirus 8 ORF73 antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IHC, WB
Immunogen HHV8 antibody was raised in mouse using recombinant human ORF73/HHV8 (122-329aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of HHV8 antibody (10R-1142)

Transfected and non-transfected 293T cell lysates were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti- ORF73/HHV8 (1:1,000) and anti-MBP (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT4C11
Isotype IgG2a kappa
Source Anti-human ORF73/HHV8 antibody, clone AT4C11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ORF73/HHV8 protein.
Method of Purification HHV8 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:1,000-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance ORF73 protein of human herpesvirus 8 (HHV-8) is a kaposi's sarcoma-associated herpesvirus (kSHV) latent protein which has been suspected of playing a crucial role in modulating viral and cellular gene expression. ORF73 is associated with cellular chromatin and stays on the chromosomes during cell division. It maintains the viral genomes during cell division by tethering the viral episomes to the chromosomes. It binds directly to replication origin recognition complexes (ORCs) that are primarily associated with the terminal repeat (TR) region of the HHV-8 genome.

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Images

  • Western Blot analysis of HHV8 antibody (10R-1142) | Transfected and non-transfected 293T cell lysates were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti- ORF73/HHV8 (1:1,000) and anti-MBP (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: €277.55
Size: 100 ul
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