HIF1a protein (His tag) (80R-1492)

Purified recombinant Human HIF1a protein

Synonyms HIF1a, HIFa-1 protein, PASD8 protein, HIFa 1 protein, HIFa 1, BHLHE78 protein, Hypoxia-inducible factor 1-alpha, HIFa-1, MOP1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of HIF1a protein (His tag) (80R-1492)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-85 amino acids: MGSSHHHHHH SSGLVPRGSH MEGAGGANDK KKISSERRKE KSRDAARSRR SKESEVFYEL AHQLPLPHNV SSHLDKASVM RLTISYLRVR KLLDAGDLDI EDDMK
Expression System E.coli
Grade & Purity > 80% pure
Molecular Weight 11.8 kDa (105aa), confirmed by MALDI-TOF (molecular size on SDS-PAGE will appear higher)
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris buffer, pH 8.0, containing 20% glycerol, 1mM DTT, 0.2M NaCl and 1mM EDTA.
Concentration 0.25 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1 beta subunit and one of three subunits (Hif-1 alpha, Hif-2 alpha or Hif-3 alpha). The activation of Hif-1 alpha is closely associated with a variety of tumors and oncogenic pathways. Hif-1 alpha consists of DNA binding domain(DBD domain), dimerization domain and C-terminal regulatory domains, including two transactivation domains(TAD), an oxygen-dependent degradation(ODD) domain, and inhibitory domains. Recombinant human Hif-1 alpha (1-85) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Coomassie Blue stained SDS-PAGE of HIF1a protein (His tag) (80R-1492) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €239.92
Size: 10 ug
OR
Shipping
View Our Distributors