Recognizes endogenous levels of Histone Deacetylase 1 protein
Cross Reactivity
Mouse, Rat, Rabbit
Applications
FC, ICC, IF, IHC, WB
Immunogen
Histone Deacetylase 1 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Histone Deacetylase 1 as the immunogen
Western blot analysis using Histone Deacetylase 1 antibody (70R-49847)
Western blot analysis of Histone Deacetylase 1 expression in MCF7 (A); HeLa (B); A431 (C); Jurkat (D); NIH3T3 (E); mouse lung (F); rat heart (G) whole cell lysates.
Histone Deacetylase 1 antibody
Western blot analysis using Histone Deacetylase 1 antibody (70R-49847)
Western blot analysis of Histone Deacetylase 1 expression in MCF7 (A); HeLa (B); A431 (C); Jurkat (D); NIH3T3 (E); mouse lung (F); rat heart (G) whole cell lysates.
Immunohistochemical analysis using Histone Deacetylase 1 antibody (70R-49847)
Immunohistochemical analysis of Histone Deacetylase 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis using Histone Deacetylase 1 antibody (70R-49847)
Immunofluorescent analysis of Histone Deacetylase 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Specifications
Host
Rabbit
Method of Purification
Histone Deacetylase 1 antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
Histone Deacetylase 1 deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons.