Histone H1 antibody (70R-49842)

Purified Polyclonal Histone H1 antibody

Synonyms Polyclonal Histone H1 antibody, Anti-Histone H1 antibody, HIST1H1B antibody, H1F5 antibody, Histone H1.5 antibody, Histone H1a antibody, Histone H1b antibody, Histone H1s-3 antibody, HIST1H1D antibody, H1F3 antibody, Histone H1.3 antibody, Histone H1c antibody, Histone H1s-2 antibody, HIST1H1E antibody, H1F4 antibody, Histone H1.4 antibody, Histone H1b antibody, Histone H1s-4 antibody
Specificity Recognizes endogenous levels of Histone H1 protein
Cross Reactivity Mouse, Rat, Bovine, Rabbit
Applications ICC, IF, IHC, WB
Immunogen Histone H1 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H1 as the immunogen

Images

Western blot analysis using Histone H1 antibody (70R-49842)

Western blot analysis of Histone H1 expression in HepG2 (A); Raw264.7 (B); PC12 (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification Histone H1 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance Histone H1 is one of the five main histone protein families which are components of chromatin in eukaryotic cells. Though highly conserved, it is nevertheless the most variable histone in sequence across species.

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Images

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Western blot analysis using Histone H1 antibody (70R-49842)



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Western blot analysis of Histone H1 expression in HepG2 (A); Raw264.7 (B); PC12 (C) whole cell lysates.
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Immunohistochemical analysis using Histone H1 antibody (70R-49842)



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Immunohistochemical analysis of Histone H1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis using Histone H1 antibody (70R-49842)



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Immunofluorescent analysis of Histone H1 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Availability: In stock

Price: €197.96
Size: 100 ul
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