Histone H2A antibody (70R-11953)
Rabbit polyclonal Histone H2A antibody
Overview
Overview
| Synonyms | Polyclonal Histone H2A antibody, anti-Histone H2A antibody, H2A/X antibody, Histone HA 2 antibody, Histone HA 2, H2a/x antibody, H2AFX antibody, Histone H2A, Histone HA-2 antibody, H2A.X antibody, Histone HA-2, H2AX antibody |
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| Cross Reactivity | Human, Mouse, Rat |
| Applications | IHC, IP, WB |
| Immunogen | Histone H2A antibody was raised in Rabbit using synthetic peptide corresponding to the N-terminus of human histone H2A as the immunogen. |
| Assay Information | Histone H2A Blocking Peptide, catalog no. 33R-10838, is also available for use as a blocking control in assays to test for specificity of this Histone H2A antibody |
Images
Western Blot analysis using Histone H2A antibody (70R-11953)
Western blot analysis of Histone H2A expression with HeLa nuclear lysate
Specifications
| Host | Rabbit |
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| Isotype | IgG |
| Method of Purification | Histone H2A antibody was purified by affinity chromatography |
| Form & Buffer | Supplied in PBS buffer, pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | IP: 10-20 ug/ml, IHC: 10-30 ug/ml, WB: 0.5-4 ug/ml |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | The nucleosome is made up of four core histone proteins and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. |
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