Histone H2A antibody (70R-11953)

Rabbit polyclonal Histone H2A antibody

Synonyms Polyclonal Histone H2A antibody, anti-Histone H2A antibody, H2A/X antibody, Histone HA 2 antibody, Histone HA 2, H2a/x antibody, H2AFX antibody, Histone H2A, Histone HA-2 antibody, H2A.X antibody, Histone HA-2, H2AX antibody
Cross Reactivity Human, Mouse, Rat
Applications IHC, IP, WB
Immunogen Histone H2A antibody was raised in Rabbit using synthetic peptide corresponding to the N-terminus of human histone H2A as the immunogen.
Assay Information Histone H2A Blocking Peptide, catalog no. 33R-10838, is also available for use as a blocking control in assays to test for specificity of this Histone H2A antibody

Images

Western Blot analysis using Histone H2A antibody (70R-11953)

Western blot analysis of Histone H2A expression with HeLa nuclear lysate

Specifications

Host Rabbit
Isotype IgG
Method of Purification Histone H2A antibody was purified by affinity chromatography
Form & Buffer Supplied in PBS buffer, pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal
Concentration 500 ug/ml

Usage & Assay Information

Usage Recommendations IP: 10-20 ug/ml, IHC: 10-30 ug/ml, WB: 0.5-4 ug/ml

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The nucleosome is made up of four core histone proteins and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.

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Images

  • <div><font face=Calibri size=2>Western Blot analysis using Histone H2A antibody (70R-11953)</font></div>     | Western blot analysis of Histone H2A expression with HeLa nuclear lysate

Availability: In stock

Price: €315.75
Size: 100 ug
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