Recognizes endogenous levels of Histone H2B protein
Cross Reactivity
Mouse, Bovine, Cat, Monkey
Applications
ChIP, ICC, IF, IHC, WB
Immunogen
Histone H2B antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H2B as the immunogen
Western blot analysis using Histone H2B antibody (70R-51007)
Western blot analysis of Histone H2B expression in U2OS (A); HeLa (B); A431 (C) whole cell lysates.
Histone H2B antibody
Western blot analysis using Histone H2B antibody (70R-51007)
Western blot analysis of Histone H2B expression in U2OS (A); HeLa (B); A431 (C) whole cell lysates.
Immunohistochemical analysis using Histone H2B antibody (70R-51007)
Immunohistochemical analysis of Histone H2B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis using Histone H2B antibody (70R-51007)
Immunofluorescent analysis of Histone H2B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Specifications
Host
Rabbit
Method of Purification
Histone H2B antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the 'beads on a string' structure.