Histone H4 antibody (70R-51168)

Purified Polyclonal Histone H4 antibody

Synonyms Polyclonal Histone H4 antibody, Anti-Histone H4 antibody, H4/A antibody, H4FA antibody, H4/I antibody, H4FI antibody, H4/G antibody, H4FG antibody, H4/B antibody, H4FB antibody, H4/J antibody, H4FJ antibody, H4/C antibody, H4FC antibody, H4/H antibody, H4FH antibody, H4/M antibody, H4FM antibody, H4/E antibody, H4FE antibody, H4/D antibody, H4FD antibody, H4/K antibody, H4FK antibody, H4/N antibody, H4F2 antibody, H4FN antibody, HIST2H4 antibody, H4/O antibody, H4FO antibody, Histone H4 antibody
Specificity Recognizes endogenous levels of Histone H4 protein
Cross Reactivity Mouse, Rat, Monkey
Applications ICC, IF, IHC, WB
Immunogen Histone H4 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H4 as the immunogen

Images

Western blot analysis using Histone H4 antibody (70R-51168)

Western blot analysis of Histone H4 expression in HeLa (A); NIH3T3 (B); PC12 (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification Histone H4 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance Histone H4 plays a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

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Images

  • 

Western blot analysis using Histone H4 antibody (70R-51168)



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Western blot analysis of Histone H4 expression in HeLa (A); NIH3T3 (B); PC12 (C) whole cell lysates.
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Immunohistochemical analysis using Histone H4 antibody (70R-51168)



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Immunohistochemical analysis of Histone H4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis using Histone H4 antibody (70R-51168)



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Immunofluorescent analysis of Histone H4 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Availability: In stock

Price: €197.96
Size: 100 ul
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