HIV1 tat protein (30-AH85)
Purified recombinant HIV1 tat protein
Overview
Overview
| Synonyms | HTATIP1 protein, HIV1 tat, Transactivating regulatory protein protein , HIV 1 tat protein, PLIP protein, HIV-1 tat protein, Human tat interactive protein mRNA complete cds protein, HIV-1 tat protein, HIV 1 Tat interacting protein protein, 2interacting protein protein 60 kDa Tat interactive protein protein cPLA protein cPLA2 protein EC 2.3.1.48 protein ESA1 protein Histone acetyltransferase HTATIP protein HIV 1 Tat interacting protein 60kDa protein, HIV 1 tat, TIP60 protein, Tat interactive protein (60kD) protein, protein Tat protein, TIP protein, HIV-1 tat, HIV 1 tat protein |
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| Species | Viral |
| Protein Type | Recombinant |
| Applications | ELISA |
Specifications
| Expression System | E.coli |
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| Grade & Purity | > 99% pure |
| Method of Purification | HIV1 tat protein was purified by Ion affinity and reverse phase HPLC. |
| Form & Buffer | Supplied as a frozen liquid. Dilute tat stock solution in 150mM NaCl, 50mM Sodium Citrate, pH 6.5 immediately before use. Tat readily oxidizes in buffer solutions, which may change its LTR dependent transcriptional activation activity. |
| Concentration | 1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | Optimal conditions to be determined by end user |
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| Bioactivity | Tat concentrations in 1 ug/ml range produced at least 25 fold increase in CAT activity. The biological activity of this protein was determined by LTR-CAT activation in scrapie-loaded HeLe Cells, and in U373 MG cells in the presence of 100uM chlorochine di |
Storage & Safety
| Storage | Aliquot and store at -70 deg C or lower. Avoid repeated freeze/thaw cycles |
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General Information
| Biological Significance | Human immunodeficiency virus (HIV) is a lentivirus that causes acquired immunodeficiency syndrome (AIDS), a condition in humans in which the immune system begins to fail, leading to life-threatening opportunistic infections. HIV has several major genes coding for structural proteins that are found in all retroviruses, and several nonstructural ("accessory") genes that are unique to HIV. |
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