HLA DRB3 antibody (70R-12874)

Affinity purified Rabbit polyclonal HLA DRB3 antibody

Synonyms Polyclonal HLA DRB3 antibody, Anti-HLA DRB3 antibody, MHC class II antigen DR beta 3 chain antibody, HLA class II histocompatibility antigen DR beta 3 chain antibody, HLA-DRB1 antibody, HLA DRB3, HLA DRB-3 antibody, MHC class II antigen DRB3 antibody, HLA-DR3B antibody, MGC117330 antibody, MHC class II HLA-DR beta 3 chain antibody, HLA DRB 3 antibody, HLA DRB 3, HLA-DRB3 antibody, HLA DRB-3
Cross Reactivity Human
Applications ICC, IF, WB
Immunogen HLA DRB3 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 4 and 266 of HLA-DRB3

Images

Immunofluorescent staining using HLA DRB3 antibody (70R-12874)

Immunofluorescence analysis of methanol-fixed A549, using HLA-DRB3 antibody at 1:500 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification HLA DRB3 antibody was purified by antigen-affinity chromatography
Molecular Weight 30 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using HLA DRB3 antibody (70R-12874)</font></div>     | Immunofluorescence analysis of methanol-fixed A549, using HLA-DRB3 antibody at 1:500 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using HLA DRB3 antibody (70R-12874)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: A549) using HLA DRB3 antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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