HLADR beta antibody (70R-12638)
Affinity purified Rabbit polyclonal HLADR beta antibody
Overview
Overview
| Synonyms | Polyclonal HLADR beta antibody, Anti-HLADR beta antibody, MHC class II antigen DRB4 antibody, DR-13 antibody, DR14 antibody, leukocyte antigen antibody, HLA-DRB4 antibody, DR13 antibody, MHC class2 antigen antibody, MHC HLA DR-beta chain antibody, human leucocyte antigen DRB4 antibody, DR-14 antibody, HLA-DR4B antibody, major histocompatibility complex class II DR beta 4 antibody, HLA class II histocompatibility antigen DR beta 4 chain antibody, DRB4 antibody |
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| Cross Reactivity | Human |
| Applications | WB |
| Immunogen | HLADR beta antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 266 of HLA DR beta |
Images
Western blot analysis using HLADR beta antibody (70R-12638)
Western blot analysis of 30 ug whole cell lysate (A:Raji) using a 12 % SDS PAGE gel and HLADR beta antibody at a dilution of 1:1000
Specifications
| Host | Rabbit |
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| Isotype | IgG |
| Method of Purification | HLADR beta antibody was purified by antigen-affinity chromatography |
| Molecular Weight | 30 kDa |
| Form & Buffer | Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative. |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Usage Recommendations | WB: 1:500-1:3000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. |
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