HMGB1 protein (30R-1150)
Purified recombinant Human HMGB1 protein
Overview
Overview
| Synonyms | High-mobility group box1 protein, Sulfoglucuronyl carbohydrate binding protein protein, HMGB1 protein, HMGB 1 protein, Amphoterin protein, HMGB-1, HMG1 protein, High-mobility group box1 protein, HMG3 protein, HMGB 1, HMGB-1 protein, SBP-1 protein, HMGB1, high-mobility group (nonhistone chromosomal) protein 1 protein, DKFZp686A04236, high mobility group protein 1 protein |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of HMGB1 protein (30R-1150)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-215 amino acids: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH |
|---|---|
| Expression System | Baculovirus |
| Grade & Purity | > 90% pure |
| Molecular Weight | 25 kDa (223aa), confirmed by MALDI-TOF. |
| Form & Buffer | Supplied as a liquid in 20mM Tris pH 8.0, containing 1mM EDTA, 0.5mM DTT, and 10% glycerol. |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Endotoxin Levels | < 1.0 EU per ug of protein (determined by LAL method) |
General Information
| Biological Significance | High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor. Recombinant human HMGB1 was expressed in High Five insect cells and purified by conventional chromatography techniques. |
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